פלטות אלייזה 96 בארות HIGH BINDING תחתית שטוחה+מכסים
ELISA Plates
* GPPS and HIPS (High Impact Polystyrene)
* Conforming to USP CLASS VI
* Unique surface treatment process for higher protein adsorption properties
* 2 binding forces available: High binding force (300~400ng/cm²), and moderate binding force (200~300ng/cm²)
* 8-well and 12-well strips are provided to match the ELISA plates and for better cost-effectiveness
* Designed with a flat bottom and divided into removable and non-removable structures, to satisfy different experimental applications
* Even well diameter and thickness, ensuring high experimental accuracy and repeatability
* Transparent plate, with a CV value <5%, higher measurement flexibility, widely used in colorimetric determination
* Clearly marked with letters and numbers, to distinguish the samples in different wells
* Dimensions conform to SBS international standards, and are stable for most brands of ELISA equipment
* Sterilized by irradiation, SAL 10<span "="">-6
* DNase/RNase-free, non-pyrogenic
High binding force ELISA plate
>The plate undergoes surface treatment to increase protein binding force, up to 300~400ng/cm² (IgG)
molecular weight of binding protein: >10kD. This type of ELISA plate can improve sensitivity and reduce the coing protein concentration and usage.
If they were not present, the non-ionic detergent to fail to blocking the binding protein,
nonspecific reactions could occur, meaning the protein would need to be used as a blocking agent.
Moderate binding force ELISA plate
The ELISA plate binds with proteins through hydrophobic bonds on the surface, and is suitable for use as a solid phase carrier to macromolecule proteins with a molecular weight >20kD, protein binding capability is 200-300ng /cm²(IgG). As the ELISA plate only displays macromolecular binding, it is also suitable as a solid phase carrier for unpurified antibodies or antigens. Proteins or non-ionic detergents can be used as blocking liquid on these plates
Type of ELISA Plate | Transmittance Variation (CV) | Binding Action | Sample Characteristics | Recommended Sealing Agent |
High binding force plate 300 ~ 400ng/cm² (IgG) |
<5.00% | Hydrophobic bond | Middle/macromolecular protein with positive charge >10kD |
PBS containing 0.3% Tween 20 , combination of 0.05% Tween 20 and 1%BSA |
Moderate binding force plate 200 ~ 300ng/cm² (IgG) |
Hydrophobic bond/ionic bond | Macromolecular protein >20KD | Detergent Tween 20 is used in combination with protein, BSA, skim milk and seru |